3′ end – Communicable Diseases Research

Structure and dynamics of SARS-CoV-2 proofreading exoribonuclease ExoN

408

Published on 2022-02-142022-10-29
Journal: Proceedings of the National Academy of Sciences of
[Category] COVID-19, MERS, SARS,

A dual mechanism of action of AT-527 against SARS-CoV-2 polymerase

Article

Published on 2022-02-022022-10-04
Journal: Nature Communications
[Category] COVID19(2023년), MERS, SARS, 변종,

Targeting genomic SARS-CoV-2 RNA with siRNAs allows efficient inhibition of viral replication and spread

siRNA로 게놈 SARS-CoV-2 RNA를 표적화하면 바이러스 복제 및 확산을 효율적으로 억제할 수 있습니다.

AcademicSubjects/SCI00010

Published on 2022-01-112022-09-10
Journal: Nucleic Acids Research
[Category] 진단, 치료제,

[키워드] 3′ end active against acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 antiviral efficacy approach cell entry conserved conserved region Coronavirus-2 COVID-19 discontinuous transcription effective Ex vivo Frame genomic genomic RNA gRNA human lung inhibited Open reading frame open reading frame 1 ORF1 Prevent Prophylactic Region replicate Replication respiratory RNA SARS-CoV-2 SARS-CoV-2 replication SARS-CoV-2 RNA sequence Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus sgRNA sgRNAs siRNA siRNAs Spread target targeting therapy transcript Transcription transcripts translational unique Verifying Viral viral replication Viral RNA viral transcript viral transcripts virus-induced cell death
[DOI] 10.1093/nar/gkab1248 PMC 바로가기 [Article Type] AcademicSubjects/SCI00010

Highly sensitive and specific detection of the SARS-CoV-2 Delta variant by double-mismatch allele-specific real time reverse transcription PCR

Article

Published on 2021-11-292022-10-04
Journal: Journal of Clinical Virology
[Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, 치료기술, 치료제,

Structure-Based Primer Design Minimizes the Risk of PCR Failure Caused by SARS-CoV-2 Mutations

SARS-CoV-2 돌연변이로 인한 PCR 실패 위험을 최소화하는 구조 기반 프라이머 설계

Cellular and Infection Microbiology

Published on 2021-10-252022-09-11
Journal: Frontiers in Cellular and Infection Microbiology
[Category] MERS, SARS, 변종, 치료기술, 치료법, 치료제,

[키워드] 3′ end addition Affect Amino acid amino acid residue caused codon coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 test effective ENCODE evade failure false-negative golden standard Health less Mutation N protein nucleic acid nucleic acid sequence nucleocapsid nucleotide nucleotides occur pair PCR primer Primers principle Protein qPCR test Qualitative quantitative reverse-transcription PCR resulting reverse transcription-PCR RT-qPCR RT-qPCR test SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sequence significantly the SARS-CoV-2 thermal stability Tryptophan Vaccine variant variants
[DOI] 10.3389/fcimb.2021.741147 PMC 바로가기 [Article Type] Cellular and Infection Microbiology

SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation

SCOPE는 유연한 표적화 및 엄격한 CARF 리보뉴클레아제 활성화를 사용하여 유형 III CRISPR-Cas 진단을 가능하게 합니다.

Article

Published on 2021-08-192022-09-10
Journal: Nature Communications
[Category] MERS, SARS, 진단, 치료기술, 치료제,

RNA-Protein Interaction Analysis of SARS-CoV-2 5′ and 3′ Untranslated Regions Reveals a Role of Lysosome-Associated Membrane Protein-2a during Viral Infection

Research Article

Published on 2021-07-132022-10-29
Journal: mSystems
[Category] COVID-19,

[키워드] 3′ UTR 3′ end 3′ UTR 5′ UTR 5′ UTR 5′-UTR absence acute respiratory syndrome autophagic flux autophagy binding Bioinformatics analysis complex component coronavirus demonstrated expected Factor genomic RNA help Host host protein host proteins identify infected cells Interaction interaction partner involved isoform LAMP2 Lamp2a mechanism membrane metabolism overexpression Protein protein-protein interaction Proteins provide receptor reduced reducing Region Regulatory regulatory element Replication resource reveal RNA RNA virus RNA virus RNA-protein interaction network role SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV-2-infected cell SARS-CoV-2-infected cells significantly Tandem mass spectrometry These data translation initiation upregulated UTR UTRs viral genome viral infection viral replication Viral RNA virus replication Virus-host interaction
[DOI] 10.1128/mSystems.00643-21 PMC 바로가기 [Article Type] Research Article

Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues

역전사된 SARS-CoV-2 RNA는 배양된 인간 세포의 게놈에 통합될 수 있으며 환자 유래 조직에서 발현될 수 있습니다.

422

Published on 2021-05-252022-09-10
Journal: Proceedings of the National Academy of Sciences of
[Category] SARS, 유전자 메커니즘, 치료기술, 치료제,

[키워드] 3′ end absence acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 can not cellular chimeric chimeric RNAs clinical recovery Consensus contribute coronavirus COVID-19 Culture DNA duplication Evidence explain expressed fraction fusing Genome genomic integration help host cell human cell human cells Hypothesis in some infected cell Infection Infectious virus initial integration integration sites investigated LINE1 majority mechanism Patient patients PCR PCR-positive positive positive PCR test produced Prolonged recurrence reported respiratory reverse transcription RNA SARS-CoV-2 SARS-CoV-2 disease SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 RNA SARS-CoV-2 sequence SARS-CoV-2 sequences sequence severe acute respiratory syndrome Coronavirus Significance Support tissue tissues transcribed transcript Transcription transcripts Viral viral genome viral replication Viral RNA viral sequence viral sequences viral transcript viral transcripts
[DOI] 10.1073/pnas.2105968118 PMC 바로가기 [Article Type] 422

Structural basis for backtracking by the SARS-CoV-2 replication–transcription complex

SARS-CoV-2 복제-전사 복합체에 의한 역추적을 위한 구조적 기반

408

Published on 2021-05-112022-09-10
Journal: Proceedings of the National Academy of Sciences of
[Category] SARS, 치료제,

[키워드] (RdRp (RdRp) 3′ end acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Antiviral antivirals approach approaches backtracking caused cellular Combination complex coronavirus COVID-19 pandemic Critical cryo Cryo-electron microscopy electron microscopy enzyme Evidence facilitate Helicase molecular dynamics Molecular dynamics simulation nsp13 NTP nucleic acid nucleoside triphosphate nucleotide organism product Proofreading RdRP Regulatory Remdesivir replicated Replication respiratory RNA RNA polymerase RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 RdRp severe acute respiratory syndrome Coronavirus Significance single-stranded stimulate Structure structures suggested target Template the SARS-CoV-2 transcribed transcriptase Transcription Viral viral transcription virus viruses
[DOI] 10.1073/pnas.2102516118 PMC 바로가기 [Article Type] 408

Pervasive generation of non-canonical subgenomic RNAs by SARS-CoV-2

SARS-CoV-2에 의한 비정규 서브게놈 RNA의 광범위한 생성

Research

Published on 2020-12-012022-08-13
Journal: Genome Medicine
[Category] MERS, SARS, 유전자 메커니즘,

[키워드] 3′ end 5′ UTR abundance accessory protein addition analysis approaches canonical canonical sgRNA canonical sgRNAs caused Cell culture condition Consistency Coordinate coronavirus disease Coronavirus disease 2019 Coverage COVID-19 determine Direct RNA sequencing eight Evidence family Coronaviridae FIVE generate Genome highlight homology Host identify in viral increase in independent Infection information Integrative analysis junction landscape leader sequence make up nc-sgRNA nc-sgRNAs non-canonical sgRNA non-canonical sgRNAs non-canonical subgenomic RNA offer open reading frames ORF ORF1a ORFs positive-sense RNA virus Region reported Result RNA RNA virus SARS-CoV-2 SARS-CoV-2 biology SARS-CoV-2 genome SARS-CoV-2 pathogenesis SARS-CoV-2 RNA SARS-CoV-2 sgRNA SARS-CoV-2 sgRNAs SARS-CoV-2 transcriptome SARS-CoV-2 transcriptomes sequence sequences Sequencing Seven sgRNA sgRNAs subgenomic RNA subgenomic RNAs supplementary material the SARS-CoV-2 genome Transcription transcriptional activity transcriptional regulatory sequence transcriptional regulatory sequences transcriptomes TRS TRS-B TRS-L Viral viral isolate viral isolates viral RNAs was used worldwide pandemic
[DOI] 10.1186/s13073-020-00802-w PMC 바로가기 [Article Type] Research